FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118708
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8100.060.9026031
AGCGAAA15800.051.883981
GAGCAAA69950.051.7517171
CAAAAGC72850.048.533864
GCGAAAG18150.045.1285482
GCAAAAG81550.043.5352063
CGAAAGC20350.040.5890543
AGCAAAA102700.034.3722572
CCGACGC7550.032.64079145
AAAAGCA114650.031.1554765
AAAGCAG142650.028.4944026
GCTATAT17000.027.3113371
TATATAC26050.025.0324443
CTATATA21300.024.502052
ATGTCGA1500.001489500424.1819131
GCCGACC3352.1209416E-923.799976145
AGACGGT12550.023.101751145
AAGCAGG184900.022.453617
TACCATG19300.022.149467
GCGTTAA2303.1579224E-522.0532979
Produced by FastQC (version 0.11.5)