FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272836
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7950.054.7359241
GAGCAAA68100.042.7056771
AGCGAAA19550.042.290851
CGAAAGC20700.040.2492453
GCGAAAG21100.038.471432
CAAAAGC90400.031.2540664
GCAAAAG96400.029.1598283
GTTACGT5050.028.722811
AGCAAAA114150.024.381372
TCCGATA1801.5245321E-424.1591432
AAAGCAG134100.024.1491786
ACATGGT22900.023.7269955
CCGACGC7200.023.153221145
GCGATTA2202.2356555E-523.061708145
TGACGTA2852.802717E-722.877825
AAGCAGG146150.022.6537957
CGATGTT5800.022.4833765
CGTATGC2953.9053702E-722.1036518
GTCTACG3655.673428E-921.856881
AGGTCGG13450.021.0080558
Produced by FastQC (version 0.11.5)