FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACATATCAGAAACCGATTTGCAGACAATGTATTGGTAGATGACAAATT11.8867924528301887No Hit
GGCAAGGGCATACCAGAAAACCTGTTGAAGAACGAACGGGAAGGCGAAGC11.8867924528301887No Hit
CTTATGAATATGTAAAAAATTCACTTCTGGACATCATCAGAGGACAACAA11.8867924528301887No Hit
TTCCTATAGAGATCTGAGTGGCACATGCGTGTGCTTGTGTGTATGTGTGT11.8867924528301887No Hit
TTTACAGGGAATGATGCCAGGAAGTTTTACTATACAAATTGGAGATTTCT11.8867924528301887No Hit
AAATATAAATCAAAAACAATGTGTTTAAACACAAAATTTAACCTAAGACT11.8867924528301887No Hit
GCCAAAAACGTCTTACAAATATAGATTATATTAAACTATAAAACACAGAT11.8867924528301887No Hit
CAACCAACTACGCGCTAACTTTCTGACAATACCCTCGAGGAAATGTCAAG11.8867924528301887No Hit
GGGGGAGCAACAGGCAGAGGGAGAGGGAGAAGCAAGATCCCCACTGACTG11.8867924528301887No Hit
TCTCCCTGGATACCTCTTTCAAGATCCATTCCACGAATTGTTTCACAACA11.8867924528301887No Hit
TGGCATCTTTAAAAAAATAAAGTGATCTCAATGCCCAGTGGGGGGATGAA11.8867924528301887No Hit
CATAAAAGTTATATGAACGAAACTATTCTCTGTCGCTTTCAATCTGTGCC11.8867924528301887No Hit
GGAAAAATCCATAGACATATTTAAGTTTCAGAGTTGCATATTGATTGGAA11.8867924528301887No Hit
CATAAAATAAGATCAAACTATAAATCTATAAACTCAACTACAAAATCAAG11.8867924528301887No Hit
GAACAATCGAAAAAAAACAGAGCCTATTATCTTTTCACAAACCAAGAGCA11.8867924528301887No Hit
TTACAATGCCATTCCAGTCCTACGAGGCGGACCCATTCTGGATTTTTGAA11.8867924528301887No Hit
TCTATCGGCAACATCTTGTTGGTGGAGCTCCTCTCAGGAATAAAGAAATG11.8867924528301887No Hit
CTATTATAATTTTTTTCTTTTATCTTTTCTTTTTTAGTATCTGAAGAAAA11.8867924528301887No Hit
CCTAAAATCTATAATAGACCCATTCTAAGGGAAATTCCACCCAAAGAAAA11.8867924528301887No Hit
TCGAAACACGGTTAGAGTCTCTGAAAAAATACAGAGATACGCTTGGAGAA11.8867924528301887No Hit
CAACTAAAGCACTTCAGAATATAAAGTCAAAAACTCACCATCCCGCGCTC11.8867924528301887No Hit
TTCAAGATGCATTACACTTTATGTTGCGCAGCATGAGTGGCTGTGACTGT11.8867924528301887No Hit
TCCAGGGACTGTCCTGACCTATGGCCCGGGCTGCTGGAGTGCCCGTGCTC11.8867924528301887No Hit
GCTTGAGATTGGACCAGTCCATCATGGAAAAGAACATAGTACTGAAAGCG11.8867924528301887No Hit
ATACTGAATGAAGGAATGGGAAAAAAACCGAAAATCAAGGTAGGGATTGG11.8867924528301887No Hit
CTAGAGTCCCGTTTTCGTTTAAATACAAAAACCACCTATCCATGACAAAT11.8867924528301887No Hit
AAATTAAAAAAATAAAAAAACATTAAAAGCAAACACTAAAAAAGCTTGTA11.8867924528301887No Hit
ATACTTGAGTGACGTGTGCGGTTGATGGGCCACCCCCAGACTCCTTGCCC11.8867924528301887No Hit
TCTTCAAATGCAGCAGAATGTCATGCCATCCACACCAGCTGACTCTTGTG11.8867924528301887No Hit
TCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTAAAGGGT11.8867924528301887No Hit
GAACACTAAATGTAAAGAAAACAACACAATAAATGGCAACATAAATCACA11.8867924528301887No Hit
CATAACTACTTTTCACATAAACCATACCATGATAAAAAATTTTCATAACC11.8867924528301887No Hit
GCATTAAAGTCCAACGATGAGTAACCCAAATGCATTTATGACATCCAAAT11.8867924528301887No Hit
CAGTTCTAGACGCTGCAAGTCCAAGCGAGCTCAAGGTGCAGGCAGTTCAG11.8867924528301887No Hit
CTTATGAGGATGTCAAAAATGCAGATTTGGTCCTAATCGTCGCACTACAA11.8867924528301887No Hit
TCTTGAAAGAGGAATCCAGCTAGCCACTTAGAAAGACAAATACATCTCTA11.8867924528301887No Hit
ATATTGGACAAAGAGTCAACGCTGCAGTAATCGCTGACAGGTAAATCTGA11.8867924528301887No Hit
CACTAGTAGGAATAAAATCTGATTATCTCTAATGAGAGACTAGCAGCTCC11.8867924528301887No Hit
GCAAAAATCATATTAGTTAGATACTATTCACTGTCCCTTTAAAACTCTGA11.8867924528301887No Hit
GGAGGCGCTGGTATGGGAGATCGCGTTTAAAAGTTGGCATCGGTCTGGAT11.8867924528301887No Hit
CTTTAACCCCCCTGATGCCCCTTCTTGGCACACAGAATGACCACCAGAGT11.8867924528301887No Hit
ATATTAAAATTAAACAACAATTAAAAAAATTCACAAAAAAAAGACTCATA11.8867924528301887No Hit
CAAATATCTTCGAAAATATTACATCAAGCTATAAAACTGTCACGCATTAC11.8867924528301887No Hit
GATCATGTGAGGCTTCAAGGAAGGATATTTATAAAACAAGATTCTCAATC11.8867924528301887No Hit
TCGCTATACTGGTCATTAGTATGTTCATATGTCTTTTTCTTGAGGATTCA11.8867924528301887No Hit
GCAGGGGGATTGGATGAGGGAGAAGCAGGCTTTCCGAAGAGCAGGGAGAC11.8867924528301887No Hit
GAACCAAAAATAAATTATGTTAAATTAAACAGACCACGCAGCAGAAAAGC11.8867924528301887No Hit
GATTTTTACTGCCACAGCCATCTGACTGTTCTGAGGAAGCTAATTGTACA11.8867924528301887No Hit
ACAATGAGGAGGGAAAAAAATTAGAAGAAATTTAAACATTACCTTCTCAT11.8867924528301887No Hit
AGTAAAAACAATCTTGAAAAATAAAATAAAATAAACTAAAACGATAAAGA11.8867924528301887No Hit
ATCAAGCTCTCTAAACGCATAAAGAAAAACATCAATATAGCTTACACTAC11.8867924528301887No Hit
GTTTATGATTAACACATTGAAACACAATTACATATTATAATTATATGCTA11.8867924528301887No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAT50.0145.07
AGTAGGA50.0145.05
AGGAATA50.0145.08
TAGTAGG50.0145.04
CTAGTAG50.0145.03
CACTAGT50.0145.01
ACTAGTA50.0145.02
GTAGGAA50.0145.06
GGAATAA50.0145.09
TAAAGGT50.0145.0145
Produced by FastQC (version 0.11.5)