FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858183
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC209110.731618654228928No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA156760.5484603330157656No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG127240.44517793297350094No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT115800.4051525042308348No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT112510.3936416947410295No Hit
CAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGACCCAGATGAAAGC107060.37457363646764397No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG101050.3535462914725894No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG85970.3007854990390748No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA74560.26086503208506945No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG72670.25425243939943665No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG71120.24882941365196No Hit
AAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAAT66730.2334700052445907No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC66630.23312013261572126No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA62510.2187053803062995No Hit
GTGTTACTGTTTACTACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGAT59090.20673973639896395No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG58300.2039757426308952No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT56170.1965234556359757No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC53060.1856424168781355No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC52750.1845578117286402No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT52350.18315832121316233No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC51090.17874992608940715No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA50240.17577600874401675No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC48750.17056290657386178No Hit
CCTTAAGGTAATATATGTGGACTTCTCTCCTTGTCACTCCAATTTCGATG48350.16916341605838395No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG47920.16765896375424527No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG47200.16513988082638514No Hit
AGCTAATAGAGTCCTACAGACTTTTCCAATGGAACCTTCTTCCACTCCTT46400.16234089979542948No Hit
GAAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAA45470.1590870843469435No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA45460.15905209708405654No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT43030.15055019220252866No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA42770.14964052336746805No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA41780.14617678434166043No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA40210.1406837840684099No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG40210.1406837840684099No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT39490.1381647011405498No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC38880.13603047810444607No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC37930.1327066881301862No Hit
CAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGATT37570.13144714666625615No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT36330.12710872606827484No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG35100.12280529273318047No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC34890.12207056021255461No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG34340.12014626075377258No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG34280.11993633717645091No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT33790.11822196129499056No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT33560.1174172542485908No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAA33510.11724231793415606No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC33120.11587781468156517No Hit
TCATATTCAGATTTCCATTTCATCAATGAACAAGGCGAATCAATAGTAGT32600.11405847701144399No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG32190.1126239992330792No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG32090.11227412660420974No Hit
CCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAGGACGGC31820.11132947050626218No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA31720.11097959787739274No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG31680.11083964882584495No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA31590.11052476345986245No Hit
GTGCTTTAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGC31580.11048977619697549No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA30650.10723596074848953No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA30600.10706102443405478No Hit
GTAGTAAACAGTAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGA30190.10562654665568999No Hit
ACCTTAAGGAGTGCTATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT30120.10538163581548136No Hit
GTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGATTTG29990.10492680139795107No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG29970.10485682687217718No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA29370.10275759109896043No Hit
ATTAACCATTTCTGTTATCCTTTTGTCAGCAGTGATTGGATATTTCATTG29220.10223278215565623No Hit
ATTGAATACTGACTTAGCTAATAGAGTCCTACAGACTTTTCCAATGGAAC28830.10086827890306534No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA28760.10062336806285672No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA28730.10051840627419589No Hit
ACAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGACCCAGATGAAAG28720.10048341901130893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10350.096.723481
CAAGCTA4150.087.287594
GCAAAAG100050.081.978113
AAGCAGG116150.079.0306557
CAAAAGC105800.077.9268654
AGCAGGT101600.077.0177848
AGCAAAA122850.068.674952
GCAGGTC66050.060.883049
GCTAAAT5850.060.684597
AAGCTAA5850.060.684595
TTTAAAC22750.058.6009943
GCAGGTA65850.058.427189
AGCGAAA17500.057.2050251
TAAACTA23250.057.0251355
ACTATTC23800.056.0146758
GCAGGGT4950.055.623029
AGGCTCG2000.054.35935145
CTATTCA25050.054.0891389
AAAGCAG173450.053.5072986
AAACTAT25450.052.0956546
Produced by FastQC (version 0.11.5)