FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950170
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA183420.46433444636559945No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG166090.42046291678585984No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC152320.38560365756410486No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG100010.25317897710731435No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG84830.21475025125500927No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC82290.20832014824678433No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA82280.2082948328806102No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA81000.2050544660103236No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG79360.20090274595776889No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG78640.1990800395932327No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT77800.1969535488346071No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC77320.19573841125824967No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG67330.17044836045030973No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT64120.16232212790841913No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA63610.1610310442335393No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT62450.1580944617573421No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG60820.1539680570709615No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT57650.14594308599376737No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG56080.14196857350443146No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA55760.14115848178685983No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA51760.1310323353172142No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC51130.129437467248245No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT50560.12799449137632052No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA50480.12779196844692758No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT50020.12662746160291835No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC48720.12333646400028353No Hit
AGCTAATAGAGTCCTACAGACTTTTCCAATGGAACCTTCTTCCACTCCTT47420.12004546639764872No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT46190.11693167635823268No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT45890.11617221537300927No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA45100.11417230144525425No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC44540.11275464093950385No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT44100.11164076482784285No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG43250.10948895870304316No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA42730.10817255966198923No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC42070.1065017454944977No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG41670.10548913084753316No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG41140.1041474164403051No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG40490.1025019176389877No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA40410.10229939470959477No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA40380.10222344861107242No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG40080.10146398762584902No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG39900.10100831103471496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13450.068.4902341
GCAAAAG95300.064.771263
AGCAGGT87700.063.2885748
AAGCAGG112800.062.564727
CGAAAGC23800.060.2731483
AGCGAAA23850.058.088771
CAAAAGC106500.058.0283974
TAAACTA30900.052.7565235
TTTAAAC30850.052.3703353
ACTATTC30850.051.6738
GCGAAAG27250.051.3291322
AGCAAAA123850.051.0847552
AAACTAT31500.050.8322536
GTATTAA66150.048.904881
CTATTCA32950.047.721219
AGGCTCG2600.041.81793145
TATATTC83450.041.498963
TTATATT82900.041.0007132
AGGTCGT12350.040.4799356
GGTCGTT12550.039.8368537
Produced by FastQC (version 0.11.5)