FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316012
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9300.056.919031
GAGCAAA69600.044.2789461
GCGAAAG16650.043.0854382
AGCGAAA15850.043.0047231
CAAAAGC74200.039.442934
CGAAAGC18550.038.2719353
GCAAAAG88550.033.051493
AGCAAAA110800.026.6173132
AAAAGCA114300.025.9853945
AAAGCAG140950.025.081476
CGTATGC4455.456968E-1222.7921818
AAGCAGG162400.021.7686797
CGTTTCG2151.1806542E-520.2179559
TGATCCG7100.019.3874059
GACGACT3751.8265928E-719.3179234
TATACTG17750.018.7737565
TGACGTA5801.0913936E-1118.7350565
TAATGCG3151.697056E-518.3980224
GTATTAT30100.018.3090021
GTATTAC8000.018.1283191
Produced by FastQC (version 0.11.5)