FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440278
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2350.040.0906143
AGCGAAA2057.2759576E-1238.9490781
GAGCGAA1502.566594E-838.7130241
GCGAAAG2151.2732926E-1137.0995062
CTCCGGA850.002724271334.158551
TAGTACC2701.72804E-1029.5220684
GTATAAC2202.2046092E-523.0958391
TATATAC3854.274625E-1022.5885163
GTATAGC1750.003630224420.739121
CCTAGGG2857.0724072E-620.3544332
GCTAGGG1800.00429895120.142412
TATGGGT1800.00431595120.1286834
AATAGTA4001.6219929E-819.9409832
GTACCTA3101.470144E-518.7001956
GGATATA1950.00678750918.612031
TGGACTG2359.2202675E-418.5012595
TAGAAAC5107.057679E-1018.4731243
GTCCTAT3552.257466E-618.4023171
AGTACCT3552.2945715E-618.3709665
TACCTAT3252.2080296E-517.837117
Produced by FastQC (version 0.11.5)