FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA15430.20081392761067868No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14970.19482725186855862No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13860.18038114301257333No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA13110.17062025865042107No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9910.12897381870523822No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA8720.11348654885062334No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG8280.10776016335816067No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC7930.1032050839891563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAT903.6379788E-1272.465419
GTTACGT1650.070.356511
TTACGTG1650.070.278632
TACGTGT1800.064.405313
ACGTGTT2100.055.2045484
TTGTGCG1206.002665E-1154.3419764
CGATAGA400.00606491354.3419724
GGTGCGA1101.6825652E-952.698688
TAGTCGG1700.046.8863228
TGTGCGG1402.746674E-1046.578845
ACACCTA10050.046.204271
AGTCGAA951.9248928E-645.7735822
GTTATGT6950.044.890231
ACATGGT5050.044.477925
GAGCGAA1151.403605E-744.1641
CCGACTA3800.043.854934
CACCGAC4000.043.48492
CTACTGA13450.043.0992936
GCGATTA1351.0280928E-842.950848145
TATGTGT6950.042.743813