Basic Statistics
Measure | Value |
---|---|
Filename | HWJ22BGXC_n02_2597_d10_rep2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 768373 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA | 1543 | 0.20081392761067868 | No Hit |
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC | 1497 | 0.19482725186855862 | No Hit |
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG | 1386 | 0.18038114301257333 | No Hit |
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA | 1311 | 0.17062025865042107 | No Hit |
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG | 991 | 0.12897381870523822 | No Hit |
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA | 872 | 0.11348654885062334 | No Hit |
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG | 828 | 0.10776016335816067 | No Hit |
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC | 793 | 0.1032050839891563 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTGCGAT | 90 | 3.6379788E-12 | 72.46541 | 9 |
GTTACGT | 165 | 0.0 | 70.35651 | 1 |
TTACGTG | 165 | 0.0 | 70.27863 | 2 |
TACGTGT | 180 | 0.0 | 64.40531 | 3 |
ACGTGTT | 210 | 0.0 | 55.204548 | 4 |
TTGTGCG | 120 | 6.002665E-11 | 54.341976 | 4 |
CGATAGA | 40 | 0.006064913 | 54.341972 | 4 |
GGTGCGA | 110 | 1.6825652E-9 | 52.69868 | 8 |
TAGTCGG | 170 | 0.0 | 46.886322 | 8 |
TGTGCGG | 140 | 2.746674E-10 | 46.57884 | 5 |
ACACCTA | 1005 | 0.0 | 46.20427 | 1 |
AGTCGAA | 95 | 1.9248928E-6 | 45.773582 | 2 |
GTTATGT | 695 | 0.0 | 44.89023 | 1 |
ACATGGT | 505 | 0.0 | 44.47792 | 5 |
GAGCGAA | 115 | 1.403605E-7 | 44.164 | 1 |
CCGACTA | 380 | 0.0 | 43.85493 | 4 |
CACCGAC | 400 | 0.0 | 43.4849 | 2 |
CTACTGA | 1345 | 0.0 | 43.099293 | 6 |
GCGATTA | 135 | 1.0280928E-8 | 42.950848 | 145 |
TATGTGT | 695 | 0.0 | 42.74381 | 3 |