FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2597_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2597_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3402371
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC35200.10345726553629808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10000.041.360391
CGAAAGC15450.036.5842863
TGACGCG2653.6379788E-1232.820625145
AGCGAAA16650.032.6856231
GCGAAAG17600.032.5407872
ACTCCGA9850.032.413521
GAGCAAA69850.031.8919961
GTTACGT7400.030.3976291
ACATGGT32300.029.8354725
TACGTGT6650.029.4218963
AGTCGGC7500.028.983035
ACGTGTT7950.025.5200254
GTCGGCT8800.024.7021736
TCGGCTG9100.023.8881687
GCCGACC4303.6379788E-1223.597778145
GGTCCTA55350.023.4342549
TTACGTG8450.023.1643812
TCCGAGG15650.022.6887323
AAGCAGG115400.021.9768377
GACATGG44850.021.8102954
Produced by FastQC (version 0.11.5)