FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389036
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20000.050.7843061
GAGCAAA85550.048.422541
GAGCGAA10950.047.7034571
GCGAAAG21650.047.202982
CAAAAGC89850.045.47884
CGAAAGC23650.044.1155973
GCAAAAG100100.040.6059383
AGCAAAA114550.035.4324462
CGACGAA1301.6600045E-533.449566145
AAAAGCA138700.029.9831335
AAAGCAG167150.027.9576916
CGCAAAA2651.382341E-724.6152822
GTAGAAA27800.022.9651551
TATATAC21850.022.8801273
GTTACGT4555.456968E-1222.3227711
GCCGACC4557.2759576E-1222.29971145
AAGCAGG218300.021.639557
GAGCTAT16900.021.0066766
CAATAGG17000.020.9111841
GCTATTG17050.020.8224818
Produced by FastQC (version 0.11.5)