FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981007
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA72250.044.9909671
CGAAAGC17950.042.7915423
AGCGAAA18500.040.7893451
GCGAAAG18800.040.492432
GAGCGAA11550.039.5770761
CAAAAGC88150.036.1680454
GCAAAAG101600.031.3103083
AAAGCAG131350.028.0234136
ACATGGT21350.027.829375
AGCAAAA116500.027.5067182
TGACGCG1606.843846E-527.180933145
AAGCAGG140900.025.9706237
AAAAGCA136200.023.5675285
ACTCCGA5850.023.5658361
CCGCGTT3503.5179255E-922.78021145
TATACTG16150.022.432875
GTTGACA29250.021.8294091
GGTCCTA35800.021.4573369
GTAGCAT36350.020.7593631
TAGCATG35850.020.425592
Produced by FastQC (version 0.11.5)