FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120754
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7750.062.7082441
AGCGAAA14700.046.876781
GCGAAAG15400.045.6580122
CGAAAGC16350.042.9864923
GAGCAAA73650.038.8038671
CAAAAGC73700.038.041614
TTACGTG5250.035.8988082
GTTACGT5400.033.581321
GCAAAAG85450.032.136993
GCTATTG16050.030.2466878
GAGCTAT16850.028.3788156
TACGTGT6650.028.3289033
AGACGAC4350.026.6507073
CTACTGA32250.026.5095586
AGCAAAA104000.026.485992
GACGACT4650.026.4856954
AAAGCAG134300.024.5462766
AAAAGCA116950.024.2225465
CAATAGG19950.023.996761
CGGAGTA7450.023.3383644
Produced by FastQC (version 0.11.5)