FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496580
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9050.052.092711
GAGCAAA75200.041.762091
AGCGAAA19850.038.365511
GCGAAAG20500.037.1203422
CGAAAGC21650.035.4706573
ACTCCGA7550.034.58341
CAAAAGC104150.030.467454
ACATGGT29700.027.0773625
GCAAAAG121250.025.6925933
GCTATAT8300.025.3414841
AAGCAGG143200.025.1960477
GCCGACC4900.025.146229145
AGTCGAA3551.5643309E-1024.497932
AAAGCAG149450.023.8996126
GTCGAAG4154.5474735E-1122.6942843
AGCAAAA139900.022.0682752
ATATTCG4751.2732926E-1121.3528424
GACATGG39800.020.933224
GTAGCAT37300.020.6114831
TATATAC20450.020.5473543
Produced by FastQC (version 0.11.5)