FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243368
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA3960.1627165444922915No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3000.1232701094638572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA651.3915633E-766.937221
GACGACG701.6962262E-551.76472145
CGAAAGC1109.9815225E-846.0987133
GGTCGTT707.8523066E-441.3947567
GCGAAAG1053.8336257E-641.3947563
AGCGAAA1209.56067E-636.2353062
CCGACGC1209.56067E-636.235306145
GAGCAAA5900.034.414051
GCAAAAG5500.034.2447553
GAGCTAT1707.8374796E-834.0897986
AGCAGGT2850.033.0431868
CAAAAGC5500.032.927654
GCAGGTA1352.1475456E-532.1959239
GCCCCGT950.00471545930.5327681
AAGGTCG950.004739234730.5013985
AGGTCGT955.6084245E-430.5013986
AGAGCTA2054.0467967E-728.269595
AAAGCAG8650.025.961456
AAGCAGG9650.025.5231937
ACCATAA1450.001229616524.9795958
Produced by FastQC (version 0.11.5)