FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023465
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA59310.19616565761469046No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG52960.17516326466487955No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA42620.14096409252298275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10100.054.5771181
AGCGAAA17200.052.7108461
CGAAAGC18600.049.4650963
GCGAAAG19500.047.5740662
ATTATAT30850.038.322241
CAAAAGC90800.035.023384
TTATATT36550.034.1064072
AAGGTCG4950.033.664079
GAGCAAA100600.032.732281
AAGCAGG115550.032.352087
GCTATAA25150.032.2996981
CTATAAC24850.031.4986742
ATTCAGC35950.031.2354766
GCCGACC3500.031.063765145
GCAAAAG102200.030.6225223
TAACTAT26250.030.080915
AGCAGGT49600.029.7978788
ACTCCGA6650.029.4483181
ATATTCA41100.029.258074
GTAGAAA22200.028.4239651
Produced by FastQC (version 0.11.5)