FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4435943
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA18700.051.974911
GCGAAAG19800.050.1546972
GAGCGAA11250.046.4205781
CGAAAGC23750.043.0150833
GAGCAAA89050.038.1190871
CAAAAGC90100.036.9095274
GCAAAAG111700.029.5785543
AAAGCAG174950.022.4463486
AGCAAAA150400.022.2664172
AAAAGCA154000.022.2060765
GTAGAAA42450.021.6998441
GTATATA38500.021.2886581
GTATTAT51300.019.9357381
TTATCGC3351.32487E-619.4647254
AAGCAGG209500.018.6758297
TATATCG4354.312824E-818.3212035
TGACGCG2400.001061752618.121792145
TAGATGT39600.017.198214
GTATAAG15800.016.9853781
CGTATGC5302.2906534E-816.4051578
Produced by FastQC (version 0.11.5)