FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885190
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC14650.043.0270233
GCGAAAG15050.041.419982
AGCGAAA16600.037.1531561
GAGCAAA61650.034.837221
CAAAAGC78950.026.3370324
GAGCGAA15100.025.4673541
GCAAAAG85450.024.2501053
GTATTAT27250.023.6978191
TATATCG2303.1611125E-522.0503165
TGACGCG1650.002605173521.96514145
AAAGCAG120100.020.6923966
AAGCAGG118050.020.3766047
AGCAAAA105100.019.7247352
AAAAGCA114650.018.957975
GTATATA16800.018.5713731
CGTATGC3602.6349353E-618.114338
TAGTACC12600.017.8249474
GTTACGT3804.354999E-617.1847721
GTATAAT13550.017.1354521
ATTATAC17350.017.121593
Produced by FastQC (version 0.11.5)