FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833756
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7350.060.2147521
AGCGAAA15200.056.8020251
GCGAAAG16650.053.121272
CGAAAGC17750.049.79763
CAAAAGC65100.045.295254
GAGCAAA72600.042.5729141
GCAAAAG75000.038.9305653
AGCAAAA95300.030.8855972
AAAAGCA107250.027.3587975
AAAGCAG135950.024.8343986
GACGACT3102.4396286E-823.3710164
GTAGAAA29700.021.9860121
GCCGACC3004.57032E-721.745403145
AGACGAC3508.812458E-820.7004073
CAATAGG14850.020.5202771
CCGACTA3651.3722638E-719.8493544
AAGCAGG174300.019.745067
TATACTG18050.019.266525
GGTACCG1950.00685193318.585815145
AACAAGG51550.018.1419492
Produced by FastQC (version 0.11.5)