FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2595_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2595_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017261
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18350.045.427652
AGCGAAA18450.045.2121621
CGAAAGC18650.045.069143
GAGCGAA10450.043.7298551
GAGCAAA76050.037.3887251
CAAAAGC75400.035.938874
GCAAAAG84400.032.1950383
AGCAAAA102450.026.7447362
ACTCCGA6050.025.1777951
AAAGCAG126000.024.496456
GAGCTAT14900.021.8821726
AAAAGCA125050.021.5537765
GTATATA18900.020.724581
AAGCAGG152650.020.2672677
TAGTACC13100.019.3580174
GCGATAA1900.00587369619.088431
CAATAGG13500.017.731031
GCTATTG18050.017.2614388
GTATTAT29850.016.7671241
AGAGCTA20700.016.4509755
Produced by FastQC (version 0.11.5)