FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262415
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA15350.046.3197821
CGAAAGC15500.045.812473
GCGAAAG16200.043.849722
TAGACCG2903.3104698E-722.4860845
GTATATA26100.021.1262251
ACCATGT31800.020.9628338
CGGTGCG2556.9919086E-519.8914819
GTACGGT3001.1125558E-519.3216726
GTAGTAC8200.017.6728043
TATATCG2050.00916329317.6719915
TATATAC27550.017.6214853
GAGCGAA8300.017.4823881
ACCGCGT2500.001399669817.390048
CCATGTC36800.016.54029
GTATTAG21200.016.084621
TACGGTG4551.3827685E-615.9244577
CCAACAC39750.015.8588413
TAGTACC13000.015.6052494
ATATAGC12250.015.3789453
ACACCAT42900.015.2006186
Produced by FastQC (version 0.11.5)