FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206432
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA24550.045.5204961
GCGAAAG28450.039.7557952
CGAAAGC29200.038.7135283
TATACGA2807.2759576E-1231.0559067
GTATACG4500.025.8015161
GTATATA21550.020.2042051
ATCGTAC1900.005906092519.0703098
CGACGGG6900.018.905079
TACGAAC4354.299909E-818.3256039
TATACGG6451.8189894E-1217.9853272
ATATACG6052.0008883E-1117.9659466
ACGACGG7300.017.8686188
GCTATAT11800.017.219231
TATAATA19650.016.9727952
CTATATA15400.016.9488352
GTATAGG19050.016.37991
CCGACGC9300.016.366873145
GTGATCG8900.016.2852659
GACGACA4501.2276978E-616.10708145
TATATAC22150.016.0303613
Produced by FastQC (version 0.11.5)