FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225656
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA15800.052.8033141
CGAAAGC16350.050.5190353
TATATCG2800.046.576735
GCGAAAG19200.043.0367972
TGACGCG3001.8189894E-1128.995008145
GAGCGAA9100.023.1194251
GTCGACG2256.880182E-419.330006145
GTATTAA29950.018.4092941
CGTTATA2050.00914300317.6787872
TATTATA25600.017.2713472
CGTTAGA2952.131389E-417.1993622
TATACTG19300.016.8931145
ATATCGT8850.016.3737436
TATATAC22200.016.3186783
TTATACT13600.015.9824584
GTATAGA20000.015.9603741
TAGACCG4151.02019385E-515.7126315
AAAGCAG100100.015.5617665
ATATTAT26950.015.0747341
ATATATA29300.014.5955592
Produced by FastQC (version 0.11.5)