FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3856813
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG27850.051.2750362
CGAAAGC29350.049.1299823
AGCGAAA30900.046.2595061
TATATCG1555.525376E-528.0488855
ATACGCC4351.9808795E-919.9891173
TAGTGCG4051.8975697E-819.68097
GTATTAC10250.019.113191
TATATAC21350.017.6484743
ATATCGT4557.199742E-817.5181626
GTATATA18200.016.3457831
AGGACCG12000.016.3034115
TACGCCT5503.6119673E-815.8091654
GTATTAT32500.015.6281631
TGACGTA7102.2555469E-1015.3083695
CTGATCG11400.015.2552499
TCTATAC23500.014.8004543
ATAGTAC19300.014.6422893
GTATACG5003.6335368E-614.5118661
AAAGCAG149000.014.4432925
ACGTATG13050.014.4367727
Produced by FastQC (version 0.11.5)