FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4064437
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17550.055.7630272
AGCGAAA19200.050.6419531
CGAAAGC20900.048.5323033
TGACGCG2401.5879777E-930.201254145
GTATATA34000.023.2623961
TATATAC36450.020.8708883
CCTTATA25300.020.3435862
GAGCGAA10800.020.1560021
TTATACT32600.020.001544
TATACTG37700.019.2177495
TATATCG4553.4706318E-919.1079315
TATCGTG4303.778041E-818.534187
ATAGCGT2400.00106539118.1125054
CCTATAC15900.017.7711963
TACGCCG2050.0091667717.6709525
TATAATA39150.017.5906352
GTACGGA4155.205875E-717.4847221
CTATATA20850.017.3841572
ATATTCG4307.669096E-716.8494638
TATACCA48950.016.5771225
Produced by FastQC (version 0.11.5)