FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172464
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA12950.047.037871
GCGAAAG13700.044.957942
CGAAAGC13550.044.908453
GAGCAAA38750.027.3224281
GCAAAAG43500.023.1480083
GTATACG2202.2263892E-523.07351
TTATACT11500.021.4170274
TATACTG13900.020.8465065
GTGATCG2857.146813E-620.3368269
CAAAAGC49700.020.1141074
CCTATAC6850.020.0932923
GAGCGAA8500.018.7690331
GTTATAG6700.018.3997631
AGCAAAA57900.018.1466922
TACTATA10250.017.6735672
GTATATA17350.017.554481
GTATAAG7100.017.3631571
ATTATAC10900.017.2796483
TATATAC17950.016.9501253
CGTATGC4058.09648E-616.0996178
Produced by FastQC (version 0.11.5)