FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469417
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6350.077.69131
GCGAAAG6700.072.488222
CGAAAGC6850.070.871933
CCGACGC3650.037.73231145
GTCTATA8300.033.2156451
GAGCGAA2202.2506356E-829.6795311
CCGTCCG2001.0706426E-525.3605189
GTACGGT1902.2028547E-422.8808926
GAGCAAA24150.022.8314591
GTTACGT1600.002182863722.6433188
GTATATA8250.021.9848381
CAAAAGC24700.021.7075124
CGAACCT2053.6724415E-421.215342145
AAAGCAG52750.020.1915725
GTTATAG2908.20481E-620.0137841
TTCCGTC2205.927925E-419.7614427
AGTACGG2205.9292844E-419.7607695
GCAAAAG30200.019.6741943
GAGTGCG1850.005064673319.583419
CTATATC8450.018.8649653
Produced by FastQC (version 0.11.5)