FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351695
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC15100.044.624573
GCGAAAG15300.044.0571632
AGCGAAA18450.036.563211
GTGATCG3950.029.3500869
TACCGTA1600.002184433422.6412095
GTATATA18350.021.3459451
TAATCCG2455.1440176E-520.7005335
GTACGGA2959.529511E-619.6709671
GAGCGAA8200.019.4610631
ATACCGT1900.005912117719.0666876
CTTAGAC10800.018.1137353
TAATACT16550.017.0732484
GTATTAA20500.016.6303631
TATATAC15900.016.4048923
TATTCCG3103.1217E-416.3600985
GTATAAA13850.016.235651
CATACCG2700.002343012516.1004145
TTAGTAC8600.016.0074883
TATATAA15100.015.8402492
GTAATAC10200.015.6275373
Produced by FastQC (version 0.11.5)