FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980816
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15400.039.051352
CGAAAGC15800.038.052493
AGCGAAA17400.033.7559051
TGACGCG2704.4598382E-621.475864145
GAGCGAA8450.018.8790421
CGACGAA3151.6891503E-518.407883145
GTATTGC5601.2551027E-1018.1281721
TACACCG4506.3673724E-817.7071845
TATACTG14450.017.5456545
GTATATA23200.016.8779531
TATATAC24150.016.49713
AATACCG2650.002070761816.4011675
GTCCTAT23300.015.8718761
ATTACGC2800.002990460715.5222733
GTATTAC9650.015.0285361
TATACCA34050.014.8919065
TCTATAC25350.014.5731783
TAGTACC12250.014.1917924
CGTATAC3100.00588192214.0201173
GTATAAT15600.013.9447481
Produced by FastQC (version 0.11.5)