FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924644
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5350.048.759723
CGATACA1501.0913936E-1148.32392145
GCGAAAG5550.047.0127832
AGCGAAA5550.045.7538951
CGACGAA1453.494754E-529.994158145
CTATACG1450.001228540624.9924282
ATAGGAC5200.023.6896173
GTATTAT7000.020.7293151
ATATTAT7950.020.077451
GTATATA6500.018.9752961
TATATAA6950.018.7712922
GTATAAA6600.018.6877921
GTGTACG2000.00793194618.1185289
AAAGCAG31900.017.9461945
GAGCGAA3652.9639086E-617.8896831
ATAAGAT5701.6370905E-1017.7977713
GTATATG5401.4970283E-917.4663681
TAGGTAG3352.8692823E-517.305385
TATACAT10950.017.2066065
GTATTAA6907.2759576E-1216.8237921
Produced by FastQC (version 0.11.5)