FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161485
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC13900.046.913783
GCGAAAG14300.044.105872
AGCGAAA16300.038.280851
CAATTCG7900.029.3505469
CCGCGAC3203.4116965E-822.6434889
TATACTG22150.022.5697865
TTATACT22150.020.9342944
TATACCG2806.131968E-620.7006535
AAGGTCG6450.019.0977639
GGTCGTT5401.8189894E-1218.7842227
TACCGCG3101.4765668E-518.697667
CCTTATA20950.018.6862832
TACGGCT2801.4345309E-418.1130714
GAGCAAA49900.017.1574271
GTATATA18400.016.955921
ATACCGC4801.3267163E-716.603916
GTATTAT23650.016.5665991
TAGTACT8850.016.3733984
GAGCGAA12050.016.2572631
GTATTAC10750.016.198451
Produced by FastQC (version 0.11.5)