FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2594_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2594_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653498
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC21900.045.3203283
GCGAAAG21650.044.189282
AGCGAAA24000.041.409211
CGACGAA2301.0059011E-931.516968145
GTATATA21000.020.035321
CGTATGC3554.7306494E-516.3275058
GTATAAG13000.016.1823751
GTCCTAT26450.016.181321
TATATAC23400.015.7895933
TGACGCG2800.002976433415.533362145
TATACTG22150.015.3723665
GAGCAAA65750.015.3358071
TAATAGT19150.015.1321934
GTATAAA21550.014.8112931
TTATACT19350.014.6013924
GTCTATA15500.014.5083351
TATAATA22200.014.3648332
GTATTAA28300.014.3545361
TAGTACC16150.014.3545014
TTTATAC27300.014.33004953
Produced by FastQC (version 0.11.5)