FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547667
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC17100.052.969713
AGCGAAA16650.052.7281071
GCGAAAG17800.049.279792
GAGCGAA10050.025.9900761
GAGCAAA68850.022.6571671
GCAAAAG66450.022.2458463
CAAAAGC68800.021.8016574
TAATGCG3406.479422E-821.312224
GTCGTAC4201.2587407E-920.7301771
GTATATA31100.018.8971251
GTATAAG15650.017.1537271
AGCAAAA96700.015.51841452
AAAGCAG137100.015.3802415
GTATAGA25750.015.21554851
TAGTACT19150.015.135574
GTATACG2900.003736863815.0115091
TATACTG29050.014.7168025
CCTTATA20200.014.7141782
TTATACT20450.014.5277434
ATATAAC16200.014.3135933
Produced by FastQC (version 0.11.5)