FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308300
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19700.057.4493451
GCGAAAG23700.048.9421422
CGAAAGC25000.046.664553
GCAAAAG60000.024.7573323
TATATAC18450.024.7426053
CAAAAGC65150.023.4662114
GAGCGAA12450.023.308661
TCTATAC18750.023.1873553
GAGCAAA69550.023.157011
GTGATCG8850.022.9256959
ATAGTAC15450.021.1049963
GTATATA17900.021.0754571
GCTATAT11400.021.0007971
TATACCA25850.020.4614935
GTATAAT13800.019.9770431
CTATACC16350.019.9420474
AGCAAAA79650.019.0226862
TACGAAC2307.995698E-418.9030199
GTATAGA19650.018.0909021
ATATACC19700.018.0220764
Produced by FastQC (version 0.11.5)