FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521280
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13250.061.871621
GCGAAAG15800.053.2173582
CGAAAGC19250.043.6598553
CGACGAA1555.5133016E-528.05779145
GCGCGTA2307.9977873E-418.9023349
TTATACT17100.017.795424
TTGATCG4908.651114E-917.750849145
GAGCGAA8950.017.0225731
GTATATA25100.016.7642151
GTATAAG15150.016.281531
TAGTACT12400.015.7760094
GTATAAT16000.014.9631461
TATATAC25700.014.6597063
TATACTG22800.014.6178745
TATTATA28500.014.4971442
TACGTCG3000.004725116314.4907616
TAGTACC15500.014.4905564
AAAGCAG130250.014.3516955
GTATAGA23350.014.2922271
TGACGCG3050.005259085414.258877145
Produced by FastQC (version 0.11.5)