FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278278
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA21600.048.6977461
CGAAAGC22250.047.540893
GCGAAAG24900.042.4988172
TGATCCG4850.028.3863359
GTATATA20100.019.8500331
GTGTATA20500.018.754981
GTATTAT25900.017.9256461
TATACTA9800.017.7428325
ATTATAC18000.017.7102473
TTATACT15750.017.4797124
GTATAAC8850.017.2135561
GAGCGAA14400.016.6244011
TAAGGCG4409.756168E-716.4656374
CCGTCTA4851.4914258E-716.4341959
TATACAG23100.016.3090695
GTATTAA28650.016.2050341
TAGTACC16100.016.199734
AAAGCAG114850.015.8967135
GTATTAG13100.015.5053421
TATATAC22050.015.4430723
Produced by FastQC (version 0.11.5)