FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312908
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA14350.074.296851
GCGAAAG14850.071.243362
CGAAAGC18350.058.0338253
ATCCGTT4750.032.0286567
GATCCGT5100.031.2497046
CCGACGC7300.029.786175145
TCCGTTC7400.021.5389088
GTATAAT16600.019.6611731
TATATCG1900.005918276519.0635285
TGATCCG8800.018.9335485
TTATACT19350.017.9696884
GTATACG2901.8615344E-417.506731
TATACAG30750.017.4330525
AAAGCAG110250.017.412225
TTAGACG2500.001402032317.3856744
ACTAGCG2550.001600361217.045553
GCCGACC4307.6382275E-716.855742145
TAGGAGT18450.016.4904774
GTCTATA10550.015.8117651
GAGCGAA10550.015.8117651
Produced by FastQC (version 0.11.5)