FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3079147
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA21300.064.7078861
CGAAAGC24950.054.8883863
GCGAAAG25950.052.794662
CGACGAA1251.2675291E-534.79363145
TTGCGCG1204.0748E-430.1895625
GCGATAT1100.00970323926.347686
ATGTCCG4550.025.4803189
ACGCAAT3452.9922376E-923.1019546
AAAGCAG89900.021.7606625
GATAACG5201.8189894E-1220.9099732
CGCAATG3157.331E-720.7027597
GTATATA9250.020.3898871
TATCGCT9950.019.661145
TTAGACG4803.1832315E-1019.6225784
TTATCGC10650.019.0485694
TGACGTA5851.2732926E-1118.578195
GAAAGCA81800.018.4231534
CGACGTC3201.9330926E-518.121681145
ACGTACG3201.939588E-518.1146183
ATAACGC6052.0008883E-1117.964913
Produced by FastQC (version 0.11.5)