FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291932
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG3050.064.133545
AGCGAAA14350.044.9925161
GCGAAAG14450.043.641232
CGAAAGC16650.038.72553
TGACGCG1001.3936366E-436.243385145
GAGCGAA8000.024.4836371
GTATATA23200.024.389831
ATCGTGG8150.023.1140778
TATCGTG8750.022.3564747
CGGTGCG2653.8037742E-621.8738259
ATATCGT11150.021.4427326
TATATAC27150.017.611463
GTATAGG13850.017.2848781
TCGTGGT10150.017.13279
GTATTAT39000.017.1129681
TATAATA26150.016.6312582
TATACAG26400.016.465265
ACTATGC11500.016.3808468
GTCCTAT21500.016.1958921
GTATAAT17050.016.1681881
Produced by FastQC (version 0.11.5)