FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752552
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18700.053.107752
AGCGAAA21000.047.3322031
TATATCG2500.046.368965
CGAAAGC23100.044.540853
ATATCGT4800.022.6413976
GCTATAT14750.019.6753941
GTATTAG17400.019.1806831
GTATATA22100.019.0410611
ATATCGA4354.3120963E-818.3213146
TATAATA25550.018.1579992
TATATAC28750.018.1458283
TATACTG29650.018.0823335
ATAATAC24850.018.0778773
TTATACT22400.017.7894334
GTATTAA30450.017.631931
GTATTAT27600.017.6125071
GTATAAT19500.017.1150681
GTATAAG14950.016.9856551
CTATATA19350.016.8581732
ATAGTAC26400.016.4675673
Produced by FastQC (version 0.11.5)