FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819974
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG13500.055.831082
CGAAAGC13700.055.5253943
AGCGAAA16100.047.7633251
TATATCG3800.028.5957955
GTATATA35200.024.319461
TATACCG1700.003108621.3066715
GAGCGAA6600.020.8845081
TATATAC35050.020.2563383
ATATCGT9250.016.4470926
TATCGTG7753.6379788E-1215.8916937
ATCGTGG7402.5465852E-1115.6645138
GTATAGG11600.015.63495351
GACCGTT7452.7284841E-1115.5591777
GTATTAA51500.015.4953011
GTATAAG19200.015.4916341
GCTATAT20250.015.4048691
TATACAT56500.015.2578395
ATATCGA3859.357778E-515.07453251
TTTATAC64500.015.0510923
TATATAA40400.014.8892622
Produced by FastQC (version 0.11.5)