FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750863
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16400.044.6767651
GCGAAAG17350.043.4477422
CGAAAGC21500.035.3833473
TATATCG2507.8936864E-826.0825825
TACGAAC3702.6193447E-1023.5000139
GTATATA43350.022.7590451
TATACGA5056.2573235E-1018.6516577
GTATTAA35700.018.0853141
GTATAAT32200.018.0234661
GTATAGG12300.017.693771
ATTATAC33400.016.7029173
CCTATAC12250.016.560373
GCGATTA2700.00233633316.10766145
GTATTAT44600.016.1029191
GTATTAG22400.015.5452411
GTGTATA31750.015.3085931
ATATCGT5806.938535E-814.9905896
ATAGTAC28250.014.8750223
GTATTAC20500.014.8627651
TATTATA38800.014.5712322
Produced by FastQC (version 0.11.5)