FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733770
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19200.058.5539971
GCGAAAG22000.051.395532
CGAAAGC22600.050.0129553
GAGCGAA10500.029.012561
CGTTTCG1100.00970128226.3489159
TGATCCG6400.024.9079599
GTATATA16800.023.3136651
AACGCGC5500.021.0771567
AAACGCG5700.020.3370656
ACGCGCC9350.020.1483638
GAGCAAA64000.018.6995031
CAAAAGC61600.018.5829074
CGCGCCA10450.018.0282069
ATACCGC12500.017.388196
ATTATAC23700.017.120093
TATATAC18650.017.093863
TACCGCG2550.001599061917.04777
TATACTG23950.016.6377455
GTATAAG11150.016.2626481
ATATATC22650.015.3546323
Produced by FastQC (version 0.11.5)