FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2593_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2593_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398660
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC22550.056.553933
GCGAAAG26100.048.8847472
CGTATCG1255.2277755E-946.3749058
AGCGAAA30100.042.658791
GTATATA22300.022.1210541
GTTCGAC2908.29881E-619.988897
GAGCGAA15200.019.5678021
TATACTG20050.019.5147635
GTACCGG13150.018.7362129
GTATTAG15850.018.3076461
GTATAAG13900.018.2664851
TAACGTA4054.091853E-717.8907625
AAAGCAG119500.016.9775125
GTCTTAT17150.016.9198951
CCTATAC8700.016.6574083
ATTAACG5252.0363586E-816.5622223
TATATCG2650.002066790416.4059757
GTCCTAT16750.016.0246541
ATTATAC17450.015.7791953
AGTACCG15650.015.742288
Produced by FastQC (version 0.11.5)