FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2592_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2592_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527626
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG46280.1311930459748284No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC43970.12464473274661203No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG37100.10516987911983867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7950.048.367061
TATATCG3600.044.2755135
TATCGTG4150.041.899287
TGACGCG1401.4202669E-841.415154145
AGCGAAA13300.040.911991
GAGCAAA66800.039.099121
GCCGACC2450.038.456932145
GCGAAAG14100.038.042722
ACATGGT26600.036.770175
CGAAAGC15400.034.815523
GGTCCTA41350.032.766819
GTATTAT36100.030.1456781
GCGTTAA3151.8189894E-1229.9020449
GACATGG35150.028.237914
CAGTACG4900.028.0947769
CTAGTAC4200.027.6014963
ATCGTGG6150.027.0958218
GTTGACA38550.026.5360151
TGCGTTA3850.026.3461348
AACTGTT38600.026.277888
Produced by FastQC (version 0.11.5)