FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2592_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2592_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433797
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC37720.10984924268965231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC15350.043.901513
AGCGAAA15650.043.582621
ACTCCGA6450.042.748771
TATATCG2250.041.8658035
GAGCGAA7800.041.8617821
GCGAAAG16950.040.2083362
GAGCAAA76550.038.484041
ACATGGT28100.035.8432245
GTCGGCT8500.030.6894056
GTCGTAC1000.00605180829.024171
GGTCCTA48150.028.2930099
GCTACTA8000.027.1737029
TCGGCTG9650.027.032127
CTATATA11600.026.8762362
GTATTAT30000.026.8473571
AAGCAGG122600.026.8330657
GTCGTCT4450.026.06064145
GCTATAT10950.025.8434371
CAAAAGC119850.025.271844
GCAAAAG116600.024.9201553
Produced by FastQC (version 0.11.5)