FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312155
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATTGCTTAATTTTTCAGATGTGCCAATGATTTTACAAAATGCT62900.18990657140139877No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT48300.14582650872317268No Hit
ATATAGATGTTACATTCATGAAATAACATAATGGCATACAAAAGACTGAT46680.14093543327531471No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG44290.13371958739853662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8600.055.66931
AGCGAAA14800.055.3843461
GAGCAAA68600.050.544521
GCGAAAG16600.049.776672
CGAAAGC17600.047.749023
CAAAAGC79800.043.3013954
GCAAAAG79550.042.98543
AGCAAAA105450.032.237052
AAAAGCA120000.029.0977045
GCTATTG23000.027.7175188
TAGGCGG1050.00774093727.5971135
GAGCTAT23850.027.3367616
AAAGCAG151150.026.5039086
ATATTGC41650.024.5243246
TATATTG49550.023.8307065
TAGATGT38600.023.2713094
AAGCAGG174200.023.1220427
CTAGTAC3152.892557E-822.9989813
ATAAGAC17350.022.130723
AGTGATC38000.022.115343
Produced by FastQC (version 0.11.5)