FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3512152
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA81050.055.859621
GAGCGAA12800.048.1809541
CGAAAGC21350.039.3726353
GCGAAAG21700.039.0854452
AGCGAAA21200.038.6731031
TATATCG3000.038.646895
TTATCGC3400.038.3780172
GCAAAAG117250.038.19533
CAAAAGC118000.037.887894
TATCGCT3900.033.445853
AAAGCAG160400.031.7151746
AAGCAGG164700.030.7120297
AGCAAAA148950.029.979832
ACATGGT27650.027.7796345
AAAAGCA172000.026.288875
TAGTACT12550.024.24954
TATCGTG5200.023.6914867
GTTGACA33900.023.3288441
GCTATAT10950.023.1910461
GGTCCTA39800.022.9431329
Produced by FastQC (version 0.11.5)