FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264974
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8700.051.693751
GAGCAAA73150.044.9211
AGCGAAA16000.042.616081
GCGAAAG17500.038.928652
CGAAAGC18950.037.0842133
CAAAAGC85950.036.9194154
GCAAAAG95400.033.338243
AGCAAAA119100.026.8352972
AAAGCAG142300.025.864976
GCTATAT11150.025.3720381
AAAAGCA129950.024.6983155
TACGGAC1500.001501253324.1493424
AAGCAGG163150.023.1812347
CTATATA14850.022.9377572
GCTATTG13300.021.7905488
GTCGTCT2353.720758E-521.58952145
TATATAC22700.021.3833373
ACATGGT19050.020.9173935
GAGCTAT13950.020.7748996
TACCATG15900.020.0497387
Produced by FastQC (version 0.11.5)