FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803872
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA82350.050.2166181
GAGCGAA12400.049.146641
TATATCG2300.047.2516485
GTTACGT5500.044.8489651
ACATGGT33600.042.4796075
GCTATAT8150.040.9483571
AGCGAAA22900.040.8686641
CGAAAGC24250.038.840023
ACTCCGA7800.037.2050251
GCGAAAG26200.035.96582
GATAGCG8300.032.31298145
TACGTGT7200.032.20073
AAGCAGG156000.030.4219727
GACATGG49150.030.3662764
TTACGTG7800.029.7374062
GCATGTT47100.029.53444
CAAAAGC138900.029.4709424
CTATATA12400.028.6432362
TACCATG16900.028.2961887
TAGCATG49750.027.9740812
Produced by FastQC (version 0.11.5)