FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615412
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15700.046.629782
AGCGAAA16150.044.921461
GAGCAAA70550.041.1329081
GAGCGAA10800.040.9762761
CGAAAGC18200.040.206233
CAAAAGC86450.032.4313744
GCAAAAG87400.032.080633
TATATCG2808.272764E-925.874585
AGCAAAA115100.024.3712222
AAAGCAG148050.022.070166
AAGCAGG149150.021.568267
GTATATA28150.020.8753171
AAAAGCA144700.020.027325
TATACTG22550.019.9194135
GTATTAT42150.019.105211
TAGTGCG1950.006872975718.5761074
TTATACT23000.018.26924
GCGTTAA3653.0064166E-617.8660729
AAGGTCG4501.2322344E-616.101529
TATACAT40500.016.0997415
Produced by FastQC (version 0.11.5)